OtsAV can play an audio signature upon startup, and between songs if needed. The following options are available:
Options -> Audio Signature -> At Program Startup: Plays the OtsAV audio signature each time OtsAV is run. Un-check this option to stop the audio signature from playing each time OtsAV is run.
Tip: This option is the same as the /nosig command line option in previous versions.
Options -> Audio Signature -> Recurring -> Never: Disables playback of the audio signature between items.
You may also select between how many items the audio signature plays.
How to create a new OtsAV icon
Command Line feature